The Argentina centre-back was forced off with a dead leg in the first half of City’s 0-0 draw with Dynamo Kiev, a result [winning 3-1 on aggregate] which saw the Blues progress to the quarter-finals for the first time in their history.
Otamendi’s early exit added to an already difficult start to the game for City, who had lost captain Vincent Kompany in the opening minutes.
But while Kompany is expected to be out for at least a month, Otamendi could be back in action by the weekend to face Manchester United at the Etihad Stadium.
The 28-year-old said: “It’s very similar to a dead leg injury I have had before. I will have a test tomorrow.
“The blow wasn’t that serious but it was better not to continue and come off.
“The test, I hope it shows nothing and am hopeful I can be there on Sunday.
“That is a derby and I will have to be 100 per cent and try to win the Premier League game. It’s a derby, we want to win.”