Stephen Dobbie’s goal ensured relegated Bolton ended a 14-match winless run with victory over play-off-bound Hull.
Substitute Dobbie found the net with a neat finish from close range after the visitors failed to clear a cross.
Earlier, keeper Eldin Jakupovic denied Bolton captain Darren Pratley from six yards and debutant Brian Lenihan blocked Zach Clough’s follow-up effort.
Hull hit the woodwork twice through Robert Snodgrass and Chuba Akpom, who also sent a first-half header wide.
Snodgrass clipped the bar with a free-kick before half-time, while Akpom thundered an effort against the frame of the goal from 12 yards shortly before Dobbie’s winner on 65 minutes.
A Hull win would have given them a six-point advantage over fifth-placed Derby, but their defeat means even beating Rotherham on the final day of the regular season may not be enough to secure a home second leg in the play-offs.
Bolton, despite achieving only their fifth league victory of a disappointing campaign, are guaranteed to finish bottom of the table but they will not end the season with the lowest-ever points tally in the second tier.
That unwanted record is shared by Stockport (2001-02) and Blackpool (2014-15), who both accumulated only 26 points – four fewer than Bolton’s total of 30.